Interferon regulates c-myc gene expression in Daudi cells at the post-transcriptional level.

Interferon - a Down - regulates Insulin Receptors in Lymphoblastoid ( Daudi ) Cells

The Daudi line of human lymphoblastoid cells requires insulin and transferrin for growth in serumfree medium and is highly sensitive to the inhibitory effect of human leukocyte interferon (IFN-a) on cell proliferation. A variant subline of Daudi cells, which is resistant to the antiproliferative action of IFN-a, also has been grown in serum-free medium containing insulin and transferrin. The pr...

Increased rate of degradation of c-myc mRNA in interferon-treated Daudi cells.

The recent observation made in our laboratory that cellular myc (c-myc) mRNA has a very short half-life in a variety of normal and transformed human cells emphasized the potential importance of post-transcriptional regulation of c-myc gene expression. Jonak and Knight [Jonak, G. J. & Knight, E., Jr. (1984) Proc. Natl. Acad. Sci. USA 81, 1747-1750] have reported a selective reduction of c-myc mR...

Interferon-Resistant Daudi Cells

Insulin negatively regulates albumin mRNA at the transcriptional and post-transcriptional level in rat hepatoma cells.

Treatment of cultured H4-II-E rat hepatoma cells with insulin causes a large decrease in cytoplasmic serum albumin mRNA. This effect is observed at low doses of insulin (ED50 = 2 pM), consistent with the effect being mediated by interaction of insulin with high affinity insulin receptors. The reduction in cytoplasmic albumin mRNA is first observed 8-12 h following insulin addition, and albumin ...

Interferon-induced gene expression in wild-type and interferon-resistant human lymphoblastoid (Daudi) cells.

Interferon-induced gene expression was analyzed in wild-type and interferon-resistant Daudi cells. Two classes of alpha-interferon-induced mRNAs and proteins were observed: those that were similarly induced in both types of cell and those that were induced only in the wild-type cells. Furthermore, the level of c-myc mRNA decreased in the wild-type but not in the resistant cells. This differenti...